MicroRNA-145 Targets YES and STAT1 in Colon Cancer Cells
Written by Christopher M. Hale et al. on January 21, 2010 – 8:00 am -MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-145 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited.
Methodology/Principal FindingsTo investigate the role of miR-145 in colon cancer, we have employed a microarray based approach to identify miR-145 targets. Based on seed site enrichment analyses and unbiased word analyses, we found a significant enrichment of miRNA binding sites in the 3′-untranslated regions (UTRs) of transcripts down-regulated upon miRNA overexpression. Gene Ontology analysis showed an overrepresentation of genes involved in cell death, cellular growth and proliferation, cell cycle, gene expression and cancer. A number of the identified miRNA targets have previously been implicated in cancer, including YES, FSCN1, ADAM17, BIRC2, VANGL1 as well as the transcription factor STAT1. Both YES and STAT1 were verified as direct miR-145 targets.
Conclusions/SignificanceThe study identifies and validates new cancer-relevant direct targets of miR-145 in colon cancer cells and hereby adds important mechanistic understanding of the tumor-suppressive functions of miR-145.
Posted in Cell Biology | Comments Off
Let It Flow: Morpholino Knockdown in Zebrafish Embryos Reveals a Pro-Angiogenic Effect of the Metalloprotease Meprin α2
January 21, 2010 – 8:00 amBackground Meprin metalloproteases are thought to be involved in basic physiological ...
Comments OffHedgehog Promotes Neovascularization in Pancreatic Cancers by Regulating Ang-1 and IGF-1 Expression in Bone-Marrow Derived Pro-Angiogenic Cells
January 21, 2010 – 8:00 amBackground The hedgehog (Hh) pathway has been implicated in the pathogenesis ...
Comments OffDNA Lesions Induced by Replication Stress Trigger Mitotic Aberration and Tetraploidy Development
January 21, 2010 – 8:00 amDuring tumorigenesis, cells acquire immortality in association with the ...
Comments OffCloned Myogenic Cells Can Transdifferentiate In Vivo into Neuron-Like Cells
January 21, 2010 – 8:00 amBackground The question of whether intact somatic cells committed to a ...
Comments OffExpanding the Substantial Interactome of NEMO Using Protein Microarrays
January 20, 2010 – 8:00 amSignal transduction by the NF-kappaB pathway is a key regulator ...
Comments OffA Role for Non-Covalent SUMO Interaction Motifs in Pc2/CBX4 E3 Activity
January 20, 2010 – 8:00 amBackground Modification of proteins by the small ubiquitin like modifier (SUMO) ...
Comments OffEndogenous Morphine Levels Are Increased in Sepsis: A Partial Implication of Neutrophils
January 20, 2010 – 8:00 amBackground Mammalian cells synthesize morphine and the respective biosynthetic pathway has ...
Comments OffFunctional Exploration of the Adult Ovarian Granulosa Cell Tumor-Associated Somatic FOXL2 Mutation p.Cys134Trp (c.402C>G)
January 20, 2010 – 8:00 amBackground The somatic mutation in the FOXL2 gene c.402C>G (p.Cys134Trp) has ...
Comments OffJS-K, a Nitric Oxide Prodrug, Has Enhanced Cytotoxicity in Colon Cancer Cells with Knockdown of Thioredoxin Reductase 1
January 20, 2010 – 8:00 amBackground The selenoenzyme thioredoxin reductase 1 has a complex role relating ...
Comments Off